COFs resin in biomedical purification is a kind of material with uniform pore, adjustable size, customizable function, high specific surface area and high porosity. In biopharmaceutical manufacturing, separation and purification is a key link, involving the production of macromolecular biological drugs. As a chromatographic filler or chromatographic medium, COFs resin can effectively capture and purify target bioactive ingredients and improve the purity of drugs. The cost of separation and purification is high, so it is important to optimize the performance of COFs resin to reduce the production cost. In addition, the structure of COFs resin is stable, not easy to swell, and the function can be customized, which makes it have broad application prospects in biomedicine purification. With the deepening of research and technological progress, COFs resin will play a more important role in the field of biomedical purification.